A selection of the Man Who Shot Liberty Valance star's personal mementos went on the block at Heritage auction house on Friday.
The green cap that Wayne wore in The Green Beret netted $179,250 (£114,573) and the cowboy hat he donned for Big Jake took in $119,500 (£76,382).
Wayne's driver's license and his eye patch from the original True Grit were among more than 700 items that sold.
A portion of the proceeds from the sale will benefit the John Wayne Cancer Foundation, which was established in 1985.
Wayne died in June 1979 after a long battle with stomach cancer.
He won an Academy Award, three Golden Globes and four People's Choice Awards over the course of his 50-year career.
