The acting couple confirmed that they are now living apart following two years of marriage.
A joint statement read: "After long and careful consideration on both our parts, we've decided to end our marriage.
"We entered our relationship with love and it's with love and kindness we leave it. While privacy isn't expected, it's certainly appreciated."
Reynolds, 34, and Johansson, 26, married in a private ceremony in Tofino, BC in September 2008 after a six-month engagement.
The pair were rumoured to have been experiencing problems as a result of their competing work schedules.
However, Reynolds said in September that his marriage to Johansson was "the best part" of his life.
Prior to their relationship, the Canadian actor was engaged to singer Alanis Morissette, while Johansson dated former Black Dahlia co-star Josh Hartnett.
